Serena and Blair watch proudly as the ABC guy hands over Chuck's liquor license, but at the last second he's all, "My pleasure, I'm always happy to help out Jack Bass," and then leaves just as the music goes, What?
Blair jumps in there to distract him with more manic squawks about "the cornerstone of the new Chuck Bass Empire," and Serena shakes her head like oh girl because obviously this is Blair's doing, but then Chuck starts screaming at Serena because he doesn't know that Blair was on the original call, and has known about the opening the whole time, or about her lurking around Serena's office all day listening to all of his problems and trying to solve them in the most fucked-up ways possible. Serena just stands there waiting for Blair to man up, and finally sighs, "Blair?" Nobody moves, and Blair's like whistling Dixie and staring at the pressed-tin ceiling, so Serena half-masts her eyes and goes, "Blair. Did you call Jack?"
Chuck stares at her like he just got knifed, which he basically did, and Blair puts on her best smile: "Well, you have the liquor license. What does it matter who called who to get it?" Serena gets a headache; meanwhile, actual stormclouds form over the intense tiki anger of Chuck Bass. "Jack. Is the last person. I would call for help." Instead of forming a rational response, Blair rushes him with her arms out Frankenstyle and going back to the gay-kiss well one more time, but Chuck's had it. He straight-up tells her the gay thing is not a big deal, but the reason he refused to say the eight letters for so long is because she is INSANE and CANNOT BE TRUSTED. And here we thought that's why he loved her.
Blair makes the just-as-valid point that she only did this because she loves him, which is true enough that he doesn't flat break up with her, and instead retreats back to detached distrust: "Be that as it may. I have a club to open, and you're no longer invited." Still sort of appalled, Serena joins him at the bar, and they turn their backs on Blair, and it's awful for all three of them because now they're all on the same page: Blair tried to play Grownup Club, and failed, so now they're back to the original team, and she's on the outside.
The only two people she loves, the only two people that make her crazy, have washed their hands of her in this venture. Another test she's failed; another attempt to be a part of something, get that power back, and she demonstrated exactly how deep she was willing to dig to prove herself, and there they are, backs turned on her, telling her some people are just better than others. And she can't turn on Chuck at this point, because there's no point in making more waves, so in a second she's going to have to hate Serena. But that's for later: Right now, there's just the horror and the pain on her face, and worst of all to see, the confusion, because she honestly still doesn't understand what she did wrong.